产品英文名称 | Anti-GAPDH Rabbit Polyclonal Antibody |
产品中文名称 | GAPDH兔多克隆抗体 |
别名 | GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH |
克隆类型 | 多克隆 |
检测类型 | WB,IHC-P |
反应性 | 人,小鼠,鸡,兔,猴,大鼠,绵羊 |
宿主 | 兔 |
浓度 | 1 mg/ml |
免疫原 | 重组蛋白 |
样本类型 | WB#IHC-P |
产品形式 | 液体形式 |
基因ID | 2597 |
目标条带分子量 | 37 |
蛋白序列链接 | http://www.uniprot.org/uniprot/P04406 |
存储缓冲液 | PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
其他 | 该抗体可检测内源蛋白 |
背景 | GAPDH encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. Glyceraldehyde-3-phosphate dehydrogenase has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Glyceraldehyde-3-phosphate dehydrogenase has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, Glyceraldehyde-3-phosphate dehydrogenase contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
Fig. Western blot analysis of 293T (1, Rat brain (2, NIH 3T3 (3, Sheep Muscle (4, 兔 testis (5, diluted at 1:20000. Secondary antibody was diluted at 1:20000.
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