产品英文名称 | Luciferase Mouse Monoclonal Antibody (6B8) |
产品中文名称 | 荧光素酶小鼠单克隆抗体 (6B8) |
克隆类型 | 单克隆 |
检测类型 | WB |
反应性 | 萤火虫 |
宿主 | 小鼠 |
浓度 | 1 mg/ml |
免疫原 | 重组蛋白: Luciferase of Luciferase |
样本类型 | WB# IF# IHC-P |
产品形式 | 液体形式 |
基因ID | N/A |
目标条带分子量 | 60 |
蛋白序列链接 | http://www.uniprot.org/uniprot/P08659 |
存储缓冲液 | 含有50%甘油,0.5%BSA和0.02%叠氮化钠的PBS缓冲液。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
其他 | 该抗体可检测内源蛋白 |
背景 | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
Fig.1. Immunofluorescence analysis of human lung tissue. 1, Luciferase Mouse Monoclonal Antibody (6B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||ABM40300-2.jpg