产品英文名称 | Anti-His Tag Rabbit Polyclonal Antibody |
产品中文名称 | His标签兔多克隆抗体 |
别名 | His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag |
克隆类型 | 多克隆 |
检测类型 | WB |
宿主 | 兔 |
免疫原 | 合成多肽 |
样本类型 | WB |
产品形式 | 液体形式 |
存储缓冲液 | PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
背景 | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
Fig. Western blot analysis of His fusion protein in cell lysate from transfected 293T cells (lane A) and baculovirus expression vector system (lane B) at 1:2000 dilutions.
作者:Liu D, Zhou Y, Peng Y, et al 杂志名称:Front Mol Neurosci IF:4
作者:Hao J, Li S, Shi X, et al 杂志名称:Sci Rep IF:4