产品英文名称 | Anti-TAP Tag Monoclonal Antibody (4H2) |
产品中文名称 | TAP标签单克隆抗体(4H2) |
别名 | TAP tag; Tandem Affinity Purification; Tandem Affinity Purification Tag |
克隆类型 | 单克隆 |
检测类型 | WB |
宿主 | 小鼠 |
免疫原 | 重组蛋白 |
样本类型 | WB |
产品形式 | 液体形式 |
存储缓冲液 | PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
背景 | The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB, a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes. |
Fig. Western blot analysis of TAP recombinant protein with Anti-TAP tag monoclonal Antibody (4H2) at 1:5000 dilution.
检测到的目的条带大小基于重组蛋白分子量的基础上,再加20 KD。