商品信息

商品属性

附加信息

图片及说明

Fig.1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488/PI Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).|||KTA0002-2.jpg

• 细胞样本较少时，为了防止细胞损失，不用PBS洗涤，可以吗？

  建议至少洗涤2-3次，因为培养基里面的杂质很多，会粘在细胞膜上。洗涤之后充分离心再去除上清液，小心操作，不会有细胞损失的，细胞个数在一个数量级之内都没什么影响。

• 该试剂盒是标记细胞凋亡哪个时期的试剂盒？

  标记细胞凋亡早期。

• 该试剂盒可以用来检测细胞周期吗？

  可以取出其中的PI组分对酒精固定后的细胞进行染色，并用流式细胞仪进行检测和定量。

• 在加入100ul Annexin V结合缓冲液后，可不可以等到60分钟左右再去做下一步呢?

  因为这个实验是活染，细胞先染完第一种染料，如果间隔时间太长，细胞活力就会受影响。

• 该试剂盒做流式细胞术是否要用细胞上清?

  染好之后用细胞混悬液上机即可，不需要用细胞上清。

• Annexin V-FITC细胞凋亡试剂盒是否可以单染？

  可以，只要不使用PI染料就可以了。

本产品已在(33)种出版物中被引用

• Organic extracts in PM2. 5 are the major triggers to induce ferroptosis in SH-SY5Y cells

  作者：Guo, CanCan, et al. 杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• miR-4687-5p Affects Intracellular Survival of Mycobacterium tuberculosis through Its Regulation of NRAMP1 Expression in A549 Cells

  作者：Meng C, Chen G, Liu Y 杂志名称：Microorganisms IF：4

  查看文献

• Endoplasmic reticulum stress mediated by ROS participates in cadmium exposure-induced MC3T3-E1 cell apoptosis

  作者：Jia, Lina, et al.  杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• Use of Transcriptomics to Reveal the Joint Immunotoxicity Mechanism Initiated by Difenoconazole and Chlorothalonil in the Human Jurkat T-Cell Line

  作者：Li Y C, Liu S Y, Li H R, et al 杂志名称：Foods IF：5

  查看文献

• Single-nucleus RNA Sequencing reveals the mechanism of cigarette smoke exposure on diminished ovarian reserve in mice

  作者：Li F, Wang Y, Xu M, Hu N, Miao J, Zhao Y, Wang L. 杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• Nuclear factor 90 promotes angiogenesis by regulating HIF-1α/VEGF-A expression through the PI3K/Akt signaling pathway in human cervical cancer

  作者：Zhang W, Xiong Z, Wei T, et al 杂志名称：Cell Death Dis IF：6

  查看文献

• Use of Transcriptomics to Reveal the Joint Immunotoxicity Mechanism Initiated by Difenoconazole and Chlorothalonil in the Human Jurkat T-Cell Line

  作者：Li, Yun-Cheng, et al 杂志名称：Foods IF：5

  查看文献

• miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

  作者：Wang, Xiaofei, et al. 杂志名称：Redox Biology IF：5

  查看文献

• Tyrosine phosphorylation-mediated YAP1-TFAP2A interactions coordinate transcription and trastuzumab resistance in HER2+ breast cancer

  作者：Zou H, Luo J, Guo Y, et al 杂志名称：Drug Resistance Updates IF：24

  查看文献

• Comparative Transcriptome Analysis to Investigate the Immunotoxicity Mechanism Triggered by Dimethomorph on Human Jurkat T Cell Lines

  作者：Li Y C, Liu S Y, Meng F B, et al. 杂志名称：Foods IF：6

  查看文献

• miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

  作者：Wang, Xiaofei, et al. 杂志名称：Redox Biology IF：11

  查看文献

• Establishment of insect cell lines expressing green fluorescent protein on cell surface based on AcMNPV GP64 membrane fusion characteristic

  作者：Ben-Xiang Qi, Ying-Jian Chen, et al 杂志名称：Cytotechnology IF：2

  查看文献

• Lysophosphatidic acid is associated with oocyte maturation by enhancing autophagy via PI3K-AKT-mTOR signaling pathway in granulosa cells

  作者：Liu, Jia, and Chong Wang 杂志名称：Journal of Ovarian Research IF：4

  查看文献

• Development of piperazine-and oxazine-linked pyrimidines as p65 subunit binders of NF–κB in human breast cancer cells

  作者：Ravish A, Narasimhachar B C, et al 杂志名称：Biomedicines IF：5

  查看文献

• Organic extracts in PM2. 5 are the major triggers to induce ferroptosis in SH-SY5Y cells

  作者：Guo, CanCan, et al. 杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• TIMELESS upregulates PD-L1 expression and exerts an immunosuppressive role in breast cancer

  作者：Dong, Xinrui, et al.  杂志名称：Journal of Translational Medicine IF：2

  查看文献

• Exopolysaccharide from the mice ovarian bacterium Bacillus velezensis OM03 triggers caspase-3-dependent apoptosis in ovarian cancer cells

  作者：SP Chirakkara, A Abraham 杂志名称：Journal of Applied Pharmaceutical Science IF：1

  查看文献

• TIMELESS upregulates PD-L1 expression and exerts an immunosuppressive role in breast cancer

  作者：Dong, Xinrui, et al.  杂志名称：Journal of Translational Medicine IF：7

  查看文献

• High Expression of LINC01268 is Positively Associated with Hepatocellular Carcinoma Progression via Regulating MAP3K7

  作者：Jin X, Fu W, Li D, Wang N, Chen J, Zeng Z, Guo J, Liu H, Zhong X, Peng H, Yu X, Sun J, Zhang X, Wang X, Xu B, Lin Y, Liu J, Kutter C, Li Y 杂志名称：Onco Targets Ther IF：3

  查看文献

• Implications of CRNDE in prognosis, tumor immunity, and therapeutic sensitivity in low grade glioma patients

  作者：Yang, Chen, et al. 杂志名称：Cancer Cell International IF：6

  查看文献

• CUDC-907 exhibits potent antitumor effects against ovarian cancer through multiple in vivo and in vitro mechanisms

  作者：Wang Y, Wen J, Sun X, et al 杂志名称：Cancer Chemotherapy and Pharmacology IF：3

  查看文献

• Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer's disease-like neuropathology and cognitive deficits

  作者：Lei, L., Luo, Y., Kang, D., Yang, F., Meng, D., Wang, J. Z., ... & Li, H. L 杂志名称：Aging (Albany NY) IF：

  查看文献

• Cadmium exposure induces pyroptosis of TM4 cells through oxidative stress damage and inflammasome activation

  作者：Zhou J, Zhang Y, Zeng L 杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• Implications of CRNDE in prognosis, tumor immunity, and therapeutic sensitivity in low grade glioma patients

  作者：Yang, Chen, et al. 杂志名称：Cancer Cell International IF：4

  查看文献

• The detrimental effect of iron on OA chondrocytes: Importance of pro‐inflammatory cytokines induced iron influx and oxidative stress

  作者：Jing X, Du T, Li T 杂志名称：J Cell Mol Med IF：4

  查看文献

• Lysophosphatidic acid is associated with oocyte maturation by enhancing autophagy via PI3K-AKT-mTOR signaling pathway in granulosa cells

  作者：Liu, Jia, and Chong Wang 杂志名称：Journal of Ovarian Research IF：4

  查看文献

• Circular RNA SMARCA5 functions as an anti-tumor candidate in colon cancer by sponging microRNA-552

  作者：Shiwei Yang, Shanyu Gao, Tongming Liu 杂志名称：Cell Cycle IF：4

  查看文献

• High Expression of LINC01268 is Positively Associated with Hepatocellular Carcinoma Progression via Regulating MAP3K7

  作者：X Jin, W Fu, D Li 杂志名称：Onco Targets Ther IF：3

  查看文献

• Nanoparticle-based Olaparib delivery enhances its effect, and improves drug sensitivity to cisplatin in triple negative breast cancer

  作者：Zhang S, Li E, Liu Z, et al. 杂志名称：Journal of Drug Delivery Science and Technology IF：5

  查看文献

• Endoplasmic reticulum stress mediated by ROS participates in cadmium exposure-induced MC3T3-E1 cell apoptosis

  作者：Jia, Lina, et al.  杂志名称：Ecotoxicology and Environmental Safety IF：2

  查看文献

• ATF4/NUPR1 axis promotes cancer cell survival and mediates immunosuppression in clear cell renal cell carcinoma

  作者：Lu Y, Chen W, Xuan Y, et al. 杂志名称：Research Square IF：

  查看文献

• Exopolysaccharide from the mice ovarian bacterium Bacillus velezensis OM03 triggers caspase-3-dependent apoptosis in ovarian cancer cells

  作者：SP Chirakkara, A Abraham 杂志名称：Journal of Applied Pharmaceutical Science IF：1

  查看文献

• Exosomes derived from endothelial progenitor cells ameliorate glyoxylate deprivation (OGD)-induced neuronal apoptosis by delivering miR-221-3p

  作者：Pan J, Wu T, Chen B, Wu H. 杂志名称：Histology and histopathology IF：2

  查看文献

• 查看所有引用文献
• 发文章得奖励
• 在线留言