| 产品英文名称 | IPKine™ Anti-GFP Magnetic IP Kit |
| 产品中文名称 | IPKine™ GFP标签蛋白免疫沉淀试剂盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 样本类型 | IP |
| 试剂盒组分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-GFP Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
| 特点&优势 | • 高效：特异性强、靶蛋白结合量高，≥0.6 mg GFP标签融合蛋白/mL磁珠； • 通用：提供IP实验所需的所有必要缓冲液； • 可靠：提供阴性对照，可排除IgG本身和目的蛋白或其它特定生物分子的非特异性结合； • 灵活：本试剂盒提供两种洗脱方法（酸洗脱和SDS-PAGE Loading Buffer洗脱方法）。 |
| 保存建议 | 按各组分标签提示分开存储，保质期12个月。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. |
Figure. The immunoprecipitation effect of Anti-GFP Magnetic IP Kit used for GFP-Tag fusion protein. HEK293T cells were transfected with GFP-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by Elution Buffer. By using acid elution, only contained GFP-Tag fusion protein, did not contain heavy and light chains of antibody.
作者：Zhang, T., Shi, C., Hu, H. et al. 杂志名称：Nature communications IF：18
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