| 产品英文名称 | Akt Polyclonal Antibody |
| 产品中文名称 | Akt多克隆抗体 |
| 别名 | AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B |
| 克隆类型 | 多克隆 |
| 检测类型 | IF,IHC-P,WB,ELISA |
| 反应性 | 小鼠,人,大鼠 |
| 宿主 | 兔 |
| 浓度 | 1 mg/ml |
| 免疫原 | 合成多肽：人源Akt 非磷酸化位点为中心：Y315 |
| 样本类型 | WB#IF#IHC-P#ELISA |
| 产品形式 | 液体形式 |
| 基因ID | 207/208/10000 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P31749 |
| 存储缓冲液 | PBS缓冲液（pH 7.4），含有0.5%BSA（稳定剂），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 蓝冰运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 其他 | 该抗体可检测内源蛋白 |
| 背景 | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in AKT1 have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for AKT1. |
Fig.1. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Akt 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||ABP0059-2.jpg
作者：Y Lan, W Zeng, Y Wang, X Dong, X Shen, Y Gu 杂志名称：communications biology IF：7
作者：Y Lan, W Zeng, Y Wang, X Dong, X Shen, Y Gu 杂志名称：communications biology IF：7
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