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Fig. Hela cells stained with Abbkine Mitochondrial Membrane Potential Assay Kit (JC-1). A: Red fluorescence indicates healthy mitochondria, B: Green fluorescence indicates mitochondria in poor health (30min incubation in 20uM CCCP).

• 该试剂盒能用分光光度计检测吗？

  该试剂盒运用的是荧光定量法，不建议用分光光度计检测。

• 该试剂盒如果储存时间偏长染液吸出是无色，这应该如何处理？

  该试剂盒如果客户低温储存时间偏长，染料可能会低温析出，建议将瓶子振摇，然后水浴37度加热，混匀即可。

• 该试剂盒可以用分光光度计检测吗？

  不建议用，该试剂盒是基于线粒体跨膜电位的变化，运用JC-1进行细胞染色，区分健康和凋亡细胞，建议用流式细胞仪或者荧光显微镜进行检测。

• 如何在保证小老鼠精子的体外存活的前提下，用该试剂盒进行线粒体膜电位分析？

  用HBSS溶液培养小鼠精子，并用该缓冲液配置染色工作液，进行活细胞染色。

本产品已在(11)种出版物中被引用

• PA1b-like peptides alleviate mitochondrial dysfunction induced by glucose toxicity through interaction with VDAC1 in β-cells.

  作者：Huang, Huizhong, et al. 杂志名称：Food & Function IF：5

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• Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function.

  作者：Teng, He, et al. 杂志名称：Bioengineered IF：4

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• Proteomics-based evaluation of the mechanism underlying vascular injury via DNA interstrand crosslinks, glutathione perturbation, mitogen-activated protein kinase, and Wnt and ErbB signaling pathways induced by crotonaldehyde.

  作者：**e, Ming-Zhang, et al. 杂志名称： Clinical Proteomics IF：3

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• Mitochondrial Ca2+ overload due to altered proteostasis amplifies apoptosis in C2C12 myoblasts under hypoxia: Protective role of nanocurcumin formulation.

  作者：Kushwaha, Asha D., et al. 杂志名称：IUBMB life IF：4

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• The interplay between H19 and HIF-1α in mitochondrial dysfunction in myocardial infarction.

  作者：**e, Luhan, et al. 杂志名称：Cellular Signalling IF：4

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• Cytotoxic Effect of Escitalopram/Etoposide Combination on Etoposide-Resistant Lung Cancer.

  作者：Özkaya Gül, Serap, et al. 杂志名称：Pharmaceuticals IF：4

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• miR‐139‐5p upregulation alleviated spontaneous recurrent epileptiform discharge‐induced oxidative stress and apoptosis in rat hippocampal neurons via regulating the Notch pathway.

  作者：Zhao, Chensheng, et al. 杂志名称：Cell Biology International IF：3

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• High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis.

  作者：Wen, **gyi, et al. 杂志名称：Gut Microbes IF：12

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• Effective compound combination of Bufei Yishen formula ameliorates PM2. 5-induced COPD by inhibiting mitochondrial oxidative stress through SIRT3-mediated FOXO3 deacetylation.

  作者：Wang, **g, et al. 杂志名称：Phytomedicine IF：7

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• Ming‑Zhang **e1, Jun‑Li Liu2, Qing‑Zu Gao3, De‑Ying Bo4, Lei Wang4, **ao‑Chun Zhou1, Meng‑Meng Zhao1.

  作者：Zhang, Yu‑Chao. 杂志名称： IF：

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• Triclosan-induced neuroinflammation develops caspase-independent and TNF-α signaling pathway associated necroptosis in Neuro-2a cells.

  作者：Katiyar, Parul, et al. 杂志名称：Current Research in Toxicology IF：3

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