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图片及说明

Fig.1. Hela cells stained with Abbkine Live and Dead Cell Double Staining Kit. A: Live cells stain green, B: Dead cells (30min incubation in 70% ethanol) stain red.|||KTA1001-2.png

• 如果样本用DMSO做保护剂，是否对该试剂盒的结果有干扰？

  可以，因为我们这个试剂盒里面的染料助溶剂是DMSO，样品中的DMSO不会对试剂盒的作用有影响。

• 该试剂盒里绿色荧光和红色荧光的激发波长分别是？

  LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm)。

• 流式操作过程中设置了一个未作荧光染色的阴性对照组目的是什么？

  在流式实验中阴性对照的作用是调试流式细胞仪。如有疑问，请联系support@abbkine.com进行详细咨询。

• 试剂盒内试剂温度应该如何控制？

  建议现配现用，而且母液要一直放在-20度保存，在使用之前配成工作液并37度水浴。

• 该试剂盒能否用于水凝胶培养的细胞染色？

  采用钙黄绿素-AM (Calcein-AM) 和碘化丙啶 (PI) 试剂对细胞进行荧光染色，其中绿色荧光指示活细胞，红色荧光指示死细胞。指示活细胞的绿色荧光细胞团在水凝胶中均匀分布，指示死细胞的红色荧光基本观察不到，表明3D细胞培养水凝胶对胎儿骨髓间充质干细胞的细胞毒性很低，拥有良好的细胞相容性。

• 该试剂盒如何评价细胞存活率？

  用活细胞个数比上总细胞个数。

• 该试剂盒是标记细胞的哪种指标？

  该试剂盒分别标记细胞的两种状态，红色荧光标记的是死细胞，绿色荧光标记的是活细胞。

• 同一个细胞上能同时染2个颜色，是怎么回事？

  如果细胞染色过程中，细胞培养条件没有保持好，活细胞染料进入细胞后，细胞趋近于死亡，细胞膜缺损，导致PI进入细胞，将细胞核着色。

• 做脱落细胞的活死细胞染色，胰酶消化会不会对染色有影响？

  可以，需要进行流式仪计数，显微镜无法对脱离细胞进行观察，胰酶消化对细胞活力一般不会有影响，除非客户的细胞比较敏感

• 该试剂盒能应用于细菌染色吗？

  不能用于原核生物，细菌没有成形的细胞核。

• 该试剂盒中的LiveDye和NucleiDye应如何保存？

  请于-20℃避光保存。

本产品已在(23)种出版物中被引用

• Harnessing enucleated cancer cells as Trojan horse cell vaccines

  作者：Fang, Wenli, et al 杂志名称：Cell Reports Physical Science IF：9

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• Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury

  作者：Pei, Yanhong, et al. 杂志名称：Materials Today Advances IF：10

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• Chitosan-crosslinked polyvinyl alcohol anti-swelling hydrogel designed to prevent abdominal wall adhesion

  作者：Huang Y, Zheng J, Zeng G, et al 杂志名称：Materials Today Bio IF：8

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• Fabrication and properties of PLA/nano-HA composite scaffolds with balanced mechanical properties and biological functions for bone tissue engineering

  作者：W Wang, B Zhang, L Zhao, M Li, Y Han 杂志名称：Nanotechnology IF：4

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• Exogenous Jasmonic Acid Alleviates Blast Resistance Reduction Caused by LOX3 Knockout in Rice

  作者：Su S, Tang P, Zuo R, et al 杂志名称：Biomolecules IF：6

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• Wear Resistance and Biocompatibility of Co-Cr Dental Alloys Fabricated with CAST and SLM Techniques

  作者：W Fu, S Liu, J Jiao, Z Xie, X Huang, Y Lu, H Liu, S Hu 杂志名称：Materials (Basel) IF：3

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• Ocular surface repair using decellularized porcine conjunctiva

  作者：L Zhao, Y Jia, C Zhao 杂志名称：Acta biomaterialia IF：7

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• Programmable DNA Hydrogel Provides Suitable Microenvironment for Enhancing TSPCS Therapy in Healing of Tendinopathy

  作者：Ge, Zilu, et al.  杂志名称：Small IF：13

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• Degradation particles derived from high-purity magnesium inhibit osteogenic differentiation through regulation of macrophage polarization

  作者：Yu Sun, Han Yu, Hongzhou Peng, et al. 杂志名称：Journal of Materials Science & Technology IF：10

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• miR-4461 regulates the proliferation and metastasis of ovarian cancer cells and cisplatin resistance

  作者：Dou L, Zhang Y 杂志名称：Front Oncol IF：5

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• Light-Activated NIR-II Imaging-Guided Tumor Therapy with Enhanced HPTT/Starvation Cycle

  作者：Wu G, et al 杂志名称：Materials and Design IF：8

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• Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo

  作者：Sun Y, Chi X, Meng H, Ma M, Wang J, Feng Z, Quan Q, Liu G, Wang Y, Xie Y, Zheng Y, Peng J 杂志名称：Bioact Mater IF：9

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• Programmable DNA Hydrogel Provides Suitable Microenvironment for Enhancing TSPCS Therapy in Healing of Tendinopathy

  作者：Ge, Zilu, et al.  杂志名称：Small IF：3

  查看文献

• Harnessing enucleated cancer cells as Trojan horse cell vaccines

  作者：Fang W, **g Z, Li Y, et al 杂志名称：Cell Reports Physical Science IF：9

  查看文献

• Preparation and in vitro evaluation of a three-dimensional porous cartilage scaffold made of silk fibroin/gelatin/chitosan

  作者：M Gu, C Wang, F Tian, N An, R Hao, L Guo 杂志名称：Chinese Journal of Tissue Engineering Research IF：0

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• Grafting antibacterial polymer brushes from titanium surface via polydopamine chemistry and activators regenerated by electron transfer ATRP

  作者：Shuangshuang Wang, Jixuan Song, Yuchao Li, et al 杂志名称：Reactive and Functional Polymers IF：3

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• Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury

  作者：Pei, Yanhong, et al. 杂志名称：Materials Today Advances IF：10

  查看文献

• Three-dimensional printed multiphasic scaffolds with stratified cell-laden gelatin methacrylate hydrogels for biomimetic tendon-to-bone interface engineering

  作者：Y Cao, S Yang, D Zhao 杂志名称：Journal of Orthopaedic Translation IF：2

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• Analysis of long noncoding RNAs in Aila-induced non-small cell lung cancer inhibition

  作者：Lin Chen, Cui Wu, Heming Wang 杂志名称：Front Oncol IF：5

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• The shock of benzalkonium chloride on aerobic granular sludge system and its microbiological mechanism

  作者：Tong, Yuhao, et al. 杂志名称：Science of The Total Environment IF：5

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• Engineering an injectable gellan gum-based hydrogel with osteogenesis and angiogenesis for bone regeneration

  作者：Liu H, Li K, Guo B, et al 杂志名称：TISSUE CELL IF：3

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• Chitosan-crosslinked polyvinyl alcohol anti-swelling hydrogel designed to prevent abdominal wall adhesion

  作者：Huang Y, Zheng J, Zeng G 杂志名称：Materials Today Bio IF：8

  查看文献

• The shock of benzalkonium chloride on aerobic granular sludge system and its microbiological mechanism

  作者：Tong, Yuhao, et al. 杂志名称：Science of The Total Environment IF：10

  查看文献