产品英文名称 | ASC Polyclonal Antibody |
产品中文名称 | ASC多克隆抗体 |
别名 | PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1 |
克隆类型 | 多克隆 |
检测类型 | WB,ELISA,IF,IHC-P |
反应性 | 人,大鼠,小鼠 |
宿主 | 兔 |
浓度 | 1 mg/ml |
免疫原 | 合成多肽:人源蛋白的N末端,ASC,AA:10-90 |
样本类型 | WB#IF#IHC-P#ELISA |
产品形式 | 液体形式 |
基因ID | 29108 |
蛋白序列链接 | http://www.uniprot.org/uniprot/Q9ULZ3 |
存储缓冲液 | PBS缓冲液(pH 7.4),含有0.5%BSA(稳定剂),0.02%叠氮化钠(防腐剂)和50%甘油。 |
保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
其他 | 该抗体可检测内源蛋白 |
背景 | PYCARD encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein (PYD and CARD domain containing) is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for PYCARD. |
Fig.1. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, ASC 多克隆 Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||ABP0062-2.jpg
作者:Shao, Xiaoqi, et al 杂志名称:Journal of Ethnopharmacology IF:5