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Fig. Abbkine Protein Quantification Kit (BCA Assay) standard curve.

• 该蛋白定量试剂盒范围为50-1000 µg/ml，如果实验的蛋白浓度低于50ug/ml是否可以？有没有做过低于50ug/ml的样品验证

  该试剂盒暂时没有达到这个级别，过低浓度蛋白检测建议客户用增强型的BCA试剂盒。

• 该试剂盒中的BSA标准品浓度如何控制？

  标准品复溶时，应一次性加入足量标准品稀释液，斡旋10秒钟，静置15分钟，观察是否有沉淀析出，应用加热振摇的方法确保溶解充分。

• 该试剂盒里A和B溶液如何混溶？

  先把A稀释2倍，再按A:B=50:1稀释就行，buffer加的是和待检测样品相同的buffer。

• 该试剂盒内标准品，溶解之后存在絮状物，应该如何处理？

  建议加入足量的稀释液，用加热振摇的方法使其完全溶解。

本产品已在(22)种出版物中被引用

• Biallelic Variants in EPG5 Gene Are Associated with Parkinson's Disease.

  作者：Sun, Qi‐Ying, et al. 杂志名称： Annals of Neurology  IF：7.700

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• Ferroptosis activation contributes to kidney aging in mice by promoting tubular cell senescence.

  作者：Wang, Zheng, et al. 杂志名称：The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences  IF：

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• Intravitreal delivery of NMO-IgG causes primary retinal damage in the absence of optic nerve injury.

  作者：Chen, Biyue, et al. 杂志名称： Journal of Neuroinflammation  IF：10.100

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• Action mechanism by which gambogic acid down-regulates expression of protein C receptor to kill triple negative breast cancer stem cells.

  作者：Li, Su, et al. 杂志名称：Chinese Journal of Tissue Engineering Research IF：

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• Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling.

  作者：Cao, Tianyu, et al. 杂志名称： Molecular Neurobiology  IF：4.300

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• Novel monomers recipe derived from Shengji-Huayu formula targeting PI3K/Akt signaling pathway for diabetic wound healing based on accurate network pharmacology.

  作者：Hu, Sheng, et al. 杂志名称：Journal of Ethnopharmacology IF：5.400

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• N6‐methyladenosine‐regulated exosome biogenesis orchestrates an immunosuppressive pre‐metastatic niche in gastric cancer peritoneal metastasis.

  作者：Li, Song, et al. 杂志名称： Cancer Communications IF：24.900

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• Expression of kiwifruit-derived actinidin in Nicotiana benthamiana leaves.

  作者：Kang, Ji Hyun, Jae-Ho Lee, and Dong Wook Lee. 杂志名称： Frontiers in Plant Science  IF：4.800

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• Hydrophilic and lipophilic diblock polymer-based nanoplatform improves the bioactivity of insect growth regulator via inhibiting chitin synthesis.

  作者：Fan, Jie, et al. 杂志名称： Pesticide Biochemistry and Physiology IF：4.000

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• Effects of dietary oregano essential oil on digestive tissue structure and function, antioxidant and immune responses and gut microbiota of red swamp crayfish (Procambarus clarkii).

  作者：Guo, Xinzi, et al. 杂志名称： Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  IF：

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• β-Hydroxybutyrate-induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy.

  作者：Huo, Yihui, et al. 杂志名称：Journal of Animal Science and Biotechnology IF：6.500

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• Multiple Postnatal Sevoflurane Exposures Induce Cognitive Impairment by Disrupting Lipid Metabolism and Myelination in Mice.

  作者：Jiang, Sufang, et al. 杂志名称： Available at SSRN IF：

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• Integrated Metabolomics and Network Pharmacology to Explore the Mechanism of Huzhang Erjin Decoction against ANIT-induced Cholestatic Hepatitis.

  作者：Li, Su, et al. 杂志名称：Journal of Ethnopharmacology  IF：5.400

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• Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii).

  作者：Wang, Yintao, et al. 杂志名称：Aquatic Toxicology  IF：4.300

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• The peptide from C-Phycocyanin alleviates myocardial ischemia-reperfusion injury by suppressing ferroptosis via upregulating UCHL3.

  作者：Li, Zhaoqing, et al. 杂志名称： Free Radical Biology and Medicine IF：8.200

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• The biological effects of oral radioiodine administration on male rats (Rattus norvegicus): DNA damage and apoptosis.

  作者：Surniyantoro, Harry NE, et al. 杂志名称： Journal of Radioanalytical and Nuclear Chemistry  IF：1.600

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• Long noncoding RNA LINC01215 represses differentiation and proliferation of keratinocytes via zinc finger 652 in atopic dermatitis.

  作者：Li, Qian, et al. 杂志名称：Journal of Radiation Research and Applied Sciences IF：2.500

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• Novel monomers recipe derived from Shengji-Huayu formula targeting PI3K/Akt signaling pathway for diabetic wound healing based on accurate network pharmacology.

  作者：Hu, Sheng, et al. 杂志名称：Journal of Ethnopharmacology IF：5.400

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• MitCOM-based prognostic model identifies GLUD1 as a key suppressor of glioblastoma growth and invasion through regulation of mitochondrial structure and metabolism.

  作者：Li, Yang, et al. 杂志名称： Cancer Cell International  IF：6.000

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• Sirtuin1 deficiency could exacerbate melanocyte apoptosis under endoplasmic reticulum stress.

  作者：Zhu, Jing, et al. 杂志名称：Inflammation IF：5.000

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• Glucose release kinetics of different feed ingredients and their impact on short-term growth of pigs by influencing carbon-nitrogen supply synchronization.

  作者：Huang, Mingyi, et al. 杂志名称：Journal of Animal Science and Biotechnology IF：6.500

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• Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer.

  作者：Tang, Yu, et al. 杂志名称：Advanced Science  IF：14.100

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