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图片及说明

Fig.KTD3001 - 蛋白质定量试剂盒（BCA法）

• 标准曲线必须要减去空白孔的值吗？

  是的，空白孔（0µg/mL）记为A空，标准孔记为A标，样本孔记为A样。计算ΔA标=A标-A空，ΔA样=A样-A空

• 可以用来测可溶性蛋白吗？

  可以的，使用蛋白提取试剂盒提取蛋白后可测样本可溶性蛋白

• BCA的a液+b液配好成了墨绿色，时间一长发黑了，正常吗？

  正常的，配好之后不用会有颜色变化，这个试剂都是现配现用，配完之后立即使用

• 说明书没有写样品制备的过程，怎么制备样品？

  根据样品类型选择合适的试剂盒提取蛋白，蛋白提取后使用该试剂盒测浓度

本产品已在(41)种出版物中被引用

• Interference with SPARC inhibits Benzophenone-3 induced ferroptosis in osteoarthritis: evidence from bioinformatics analyses and biological experimentation.

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• Multiple Postnatal Sevoflurane Exposures Induce Cognitive Impairment by Disrupting Lipid Metabolism and Myelination in Mice.

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  作者：Zhu, Jing, et al. 杂志名称：Inflammation IF：5.000

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• Novel monomers recipe derived from Shengji-Huayu formula targeting PI3K/Akt signaling pathway for diabetic wound healing based on accurate network pharmacology.

  作者：Hu, Sheng, et al. 杂志名称：Journal of Ethnopharmacology IF：5.400

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• Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer.

  作者：Bashir, Mona Alrasheed, et al. 杂志名称：RSC advances IF：4

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• N6‐methyladenosine‐regulated exosome biogenesis orchestrates an immunosuppressive pre‐metastatic niche in gastric cancer peritoneal metastasis.

  作者：Li, Song, et al. 杂志名称： Cancer Communications IF：24.900

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• Expression of kiwifruit-derived actinidin in Nicotiana benthamiana leaves.

  作者：Kang, Ji Hyun, Jae-Ho Lee, and Dong Wook Lee. 杂志名称： Frontiers in Plant Science  IF：4.800

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• Fecal microbiota transplantation alleviates cognitive impairment by improving gut microbiome composition and barrier function in male rats of traumatic brain injury following gas explosion.

  作者：Dong, Xinwen, et al. 杂志名称：Frontiers in Microbiology IF：4

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• Glucose release kinetics of different feed ingredients and their impact on short-term growth of pigs by influencing carbon-nitrogen supply synchronization.

  作者：Huang, Mingyi, et al. 杂志名称：Journal of Animal Science and Biotechnology IF：6.500

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• Integrated Metabolomics and Network Pharmacology to Explore the Mechanism of Huzhang Erjin Decoction against ANIT-induced Cholestatic Hepatitis.

  作者：Li, Su, et al. 杂志名称：Journal of Ethnopharmacology  IF：5.400

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• Biallelic Variants in EPG5 Gene Are Associated with Parkinson's Disease.

  作者：Sun, Qi‐Ying, et al. 杂志名称： Annals of Neurology  IF：7.700

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• Co-localization and co-expression of Olfml3 with Iba1 in brain of mice.

  作者：Yadav, Himanshi, et al. 杂志名称：Journal of Neuroimmunology IF：3

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• Bone‐Targeted Biomimetic Nanogels Re‐Establish Osteoblast/Osteoclast Balance to Treat Postmenopausal Osteoporosis.

  作者：Cui, Yongzhi, et al. 杂志名称：Small IF：13

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• Heat stress induces IL-1β and IL-18 overproduction via ROS-activated NLRP3 inflammasome: implication in neuroinflammation in mice with heat stroke.

  作者：Du, Guoqiang, et al. 杂志名称：Neuroreport IF：2

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• Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer.

  作者：Tang, Yu, et al. 杂志名称：Advanced Science  IF：14.100

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• Inhibition of miR-4763-3p expression activates the PI3K/mTOR/Bcl2 autophagy signaling pathway to ameliorate cognitive decline.

  作者：Qi, Wenxin, et al. 杂志名称：International Journal of Biological Sciences IF：8

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• Blocking Sigmar1 exacerbates methamphetamine-induced hypertension.

  作者：Xu, Zhen-Zhen, et al. 杂志名称：Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease IF：4

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• Intravitreal delivery of NMO-IgG causes primary retinal damage in the absence of optic nerve injury.

  作者：Chen, Biyue, et al. 杂志名称： Journal of Neuroinflammation  IF：10.100

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• The peptide from C-Phycocyanin alleviates myocardial ischemia-reperfusion injury by suppressing ferroptosis via upregulating UCHL3.

  作者：Li, Zhaoqing, et al. 杂志名称： Free Radical Biology and Medicine IF：8.200

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• Accurate network pharmacology and novel ingredients formula of herbal targeting estrogen signaling for psoriasis intervention.

  作者：Wu, Xinxin, et al. 杂志名称：Journal of Ethnopharmacology IF：5

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• Ferroptosis activation contributes to kidney aging in mice by promoting tubular cell senescence.

  作者：Wang, Zheng, et al. 杂志名称：The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences  IF：

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• MitCOM-based prognostic model identifies GLUD1 as a key suppressor of glioblastoma growth and invasion through regulation of mitochondrial structure and metabolism.

  作者：Li, Yang, et al. 杂志名称： Cancer Cell International  IF：6.000

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• Zhilining Formula alleviates DSS-induced colitis through suppressing inflammation and gut barrier dysfunction via the AHR/NF-κBp65 axis.

  作者：Zhou, Rui, et al. 杂志名称：Phytomedicine IF：7

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• miR-889-3p targeting BMPR2 promotes the development of retinoblastoma via JNK/MAPK/ERK signaling.

  作者：Gao, Yuan, and Pei Du. 杂志名称：Scientific Reports IF：4

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• β-Hydroxybutyrate-induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy.

  作者：Huo, Yihui, et al. 杂志名称：Journal of Animal Science and Biotechnology IF：6.500

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• Overexpression of EspL inhibits autophagy and antigen presentation to promote the intracellular survival of Mycobacterium tuberculosis avirulent strains.

  作者：Cai, Luxia, et al. 杂志名称：Animal Diseases IF：2

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• Single-cell deconvolution algorithms analysis unveils autocrine IL11-mediated resistance to docetaxel in prostate cancer via activation of the JAK1/STAT4 pathway.

  作者：Cheng, Bisheng, et al. 杂志名称：Journal of Experimental & Clinical Cancer Research IF：11

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• Engineered microparticles modulate arginine metabolism to repolarize tumor-associated macrophages for refractory colorectal cancer treatment.

  作者：Wang, Jun, et al. 杂志名称：Journal of Translational Medicine IF：6

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• Neutrophil PPIF exacerbates lung ischemia–reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation.

  作者：Wu, Wensi, et al. 杂志名称：International Immunopharmacology IF：5

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• The biological effects of oral radioiodine administration on male rats (Rattus norvegicus): DNA damage and apoptosis.

  作者：Surniyantoro, Harry NE, et al. 杂志名称： Journal of Radioanalytical and Nuclear Chemistry  IF：1.600

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• Effects of dietary oregano essential oil on digestive tissue structure and function, antioxidant and immune responses and gut microbiota of red swamp crayfish (Procambarus clarkii).

  作者：Guo, Xinzi, et al. 杂志名称： Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  IF：

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• Unlocking the potential of N-acetylcysteine: Improving hepatopancreas inflammation, antioxidant capacity and health in common carp (Cyprinus carpio) via the MAPK/NF-κB/Nrf2 signalling pathway.

  作者：Zhu, Rui, et al. 杂志名称：Fish & Shellfish Immunology IF：4

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• Study of 7-Hydroxyflavone against Oxidative Stress in Myocardial Ischemia/Reperfusion Injury Based on Network Pharmacology and Bioinformatics.

  作者：Tan, Zhipeng, et al. 杂志名称：Yangtze Medicine IF：

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• Action mechanism by which gambogic acid down-regulates expression of protein C receptor to kill triple negative breast cancer stem cells.

  作者：Li, Su, et al. 杂志名称：Chinese Journal of Tissue Engineering Research IF：

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• Dietary chlorogenic acid supplementation protects against lipopolysaccharide-induced oxidative stress, inflammation and apoptosis in intestine of amur ide (Leuciscus waleckii).

  作者：Wang, Yintao, et al. 杂志名称：Aquatic Toxicology  IF：4.300

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• Hydroxysafflor yellow A promotes haCaT cell proliferation and migration by regulating HBEGF/EGFR and PI3K/AKT pathways and circ_0084443.

  作者：Zhang, Yue, et al. 杂志名称：Chinese journal of integrative medicine IF：2

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• Rosiglitazone promotes oligodendrocyte development and myelin formation of repeated neonatal sevoflurane exposure via PPARγ signaling.

  作者：Cao, Tianyu, et al. 杂志名称： Molecular Neurobiology  IF：4.300

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• Hydrophilic and lipophilic diblock polymer-based nanoplatform improves the bioactivity of insect growth regulator via inhibiting chitin synthesis.

  作者：Fan, Jie, et al. 杂志名称： Pesticide Biochemistry and Physiology IF：4.000

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• Investigate the efficacy of size exclusion chromatography for the isolation of extracellular vesicles from C. elegans.

  作者：Thomas, Sharon, et al. 杂志名称：Journal of Chromatography B IF：3

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• Long noncoding RNA LINC01215 represses differentiation and proliferation of keratinocytes via zinc finger 652 in atopic dermatitis.

  作者：Li, Qian, et al. 杂志名称：Journal of Radiation Research and Applied Sciences IF：2.500

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• Colquhounia root tablet improves diabetic kidney disease by regulating epithelial-mesenchymal transition via the PTEN/PI3K/AKT pathway.

  作者：Ma, Donghong, et al. 杂志名称：Frontiers in Pharmacology IF：4

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