产品英文名称 | EliKine™ Mouse IgG ELISA Kit |
产品中文名称 | EliKine™ 小鼠IgG ELISA试剂盒 |
检测类型 | 夹心法 |
反应性 | 小鼠 |
灵敏度 | 0.5 ng/mL |
样本类型 | 细胞培养上清#血清#血浆#其它生物液体 |
试剂盒组分 | •小鼠IgG微孔板 •小鼠IgG标准品•样品稀释剂 •分析缓冲液 •检测抗体 •色原体溶液 •停止溶液 •洗涤缓冲液 •板盖。 |
使用中注意事项 | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
保存建议 | 请将未开封使用的试剂盒保存于2-9℃;启用后的试剂盒,请参考说明书保存各个组分。 |
运输条件 | 蓝冰运输 |
警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
背景 | Immunoglobulin G (IgG) is a multimeric immunoglobulin, built of two heavy chains and two light chains. Each complex has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. |
Fig.1. Mouse IgG Standard Curve.|||elisa-kit.jpg