| 产品英文名称 | Human EGF protein |
| 产品中文名称 | 重组人表皮生长因子（EGF） |
| 别名 | beta-urogastrone,EGF,epidermal growth factor, hEGF, HOMG4, URG, Urogastrone |
| 序列 | Amino acid sequence derived from Human EGF protein (Met 970-Arg1023)(P01133) was expressed |
| 活性 | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.004 ng/mL. The specific activity of recombinant Human EGF is approximately >2.5 x 108IU/mg. |
| 蛋白长度 | 6.35 kDa |
| 纯度 | > 96% |
| 特点&优势 | 通过LAL法测定，每微克蛋白里内毒素含量<0.1 EU。 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的150 mM NaCl 20 mM Tris , pH 8.0。 |
| 基因ID | 1950 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P01133 |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Epidermal growth factor (EGF) is a small growth factor containing 53 amino acid residues that promotes the proliferation of mesenchymal and epidermal cells. Mature proteins, much smaller than 53aa, are produced by protein hydrolysis in the proximal EGF domain across the membrane. EGF is well preserved in mammals, and the EGF in mature humans is 70% the same as that in mice and mice. EGF and fibroblast growth factor 2 (fgf-2) induce the proliferation of neural precursor cells isolated from specific parts of the embryonic and adult brain. EGF and somatopodin c supplemental medium were substituted for 5% thrombocytopenic anemia plasma (PPP) and had the ability to inhibit BALB/C-3T3 cell density. The biological activities of EGF include epithelium formation, angiogenesis, inhibition of gastric acid secretion, proliferation of fibroblasts, and colony formation of epidermal cells in culture. |
Fig.SDS-PAGE analysis of Human EGF protein.
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