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Fig.1. 使用ExKine™ 细胞核蛋白与胞浆蛋白提取试剂盒对特定蛋白进行蛋白质印迹。样本：293T细胞裂解物；C：使用α-tubulin 抗体（A01080）分析细胞质提取物。N：使用α-tubulin抗体（A01080）分析核提取物。|||KTP3001-2.png

• 该细胞核提取试剂盒如果在下层沉淀中未检测到细胞核，而是细胞杂质，可能是什么原因？

  可能有以下三个方面原因：1. 细胞裂解不充分，导致细胞核未进入提取液中；2. 选用的离心机型号不匹配，未达到细胞核和细胞质分离的离心力；3. 操作人员动作不轻柔，EP管取放过程中有振荡，导致分层的组分重新混合在一起。

• 该试剂盒可以分别提取细胞核蛋白和胞浆蛋白吗？

  可以。

• 该试剂盒使用过程中有哪些注意事项？

  1、使用新鲜样本；2、离心时选用合适的离心力；3、取出及抽吸EP管的动作应轻柔，从大规格枪头切换成小规格枪头，吸上层液体用大规格枪头，下层液体用小规格枪头。

本产品已在(14)种出版物中被引用

• Xinshuaining preparation protects H9c2 cells from H2O2-induced oxidative damage through the PI3K/Akt/Nrf-2 signaling pathway

  作者：Han M, Lin J, Yang Y, et al. 杂志名称：Clinical and Experimental Hypertension IF：14

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• TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma

  作者：Q Luo, X Wu, Y Nan 杂志名称：Cell Reports IF：8

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• TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation

  作者：Yang, Hang, et al. 杂志名称：Molecular Medicine IF：6

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• miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia

  作者：Enbo Wang, Yunyan Zhang, Rongmei Ding 杂志名称：Mol Med Rep IF：2

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• Exendin‐4 enhances the sensitivity of prostate cancer to enzalutamide by targeting Akt activation

  作者：H Wenjing, Y Shao, Y Yu 杂志名称：The Prostate IF：

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• miR‑30a‑5p induces the adipogenic differentiation of bone marrow mesenchymal stem cells by targeting FAM13A/Wnt/β‑catenin signaling in aplastic anemia

  作者：E Wang, Y Zhang, R Ding 杂志名称：Molecular Medicine Reports IF：3

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• Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via …

  作者：Zhao X, Wang J, Li P, Tang L, Bai Y, 杂志名称：J Ophthalmol IF：1

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• HCP5 prevents ubiquitination-mediated UTP3 degradation to inhibit apoptosis by activating c-Myc transcriptional activity

  作者：Y Nan, Q Luo, X Wu, W Chang, P Zhao, S Liu, Z Liu 杂志名称：Molecular Therapy IF：12

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• HCP5 prevents ubiquitination-mediated UTP3 degradation to inhibit apoptosis by activating c-Myc transcriptional activity

  作者：Y Nan, Q Luo, X Wu, W Chang, P Zhao, S Liu, Z Liu 杂志名称：Molecular Therapy IF：12

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• Gastrodin ameliorates postoperative cognitive dysfunction in aged mice by promoting nuclear translocation of Nrf2 through activation of AMPK

  作者：Xu Y, Lu Y, Xu R 杂志名称：Brain Research IF：4

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• Casein Kinase 2-Interacting Protein-1 Alleviates High Glucose-Reduced Autophagy, Oxidative Stress, and Apoptosis in Retinal Pigment Epithelial Cells via Activating the p62/KEAP1/NRF2 Signaling Pathway

  作者：X Zhao, J Wang, P Li 杂志名称：Journal of Ophthalmology IF：3

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• TRIM37 exacerbates hepatic ischemia/reperfusion injury by facilitating IKKγ translocation

  作者：Yang, Hang, et al. 杂志名称：Molecular Medicine IF：6

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• Inhibition of USP30 Promotes Mitophagy by Regulating Ubiquitination of MFN2 by Parkin to Attenuate Early Brain Injury After SAH

  作者：Liu, Yang, et al 杂志名称：Translational Stroke Research IF：7

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• Inhibition of USP30 Promotes Mitophagy by Regulating Ubiquitination of MFN2 by Parkin to Attenuate Early Brain Injury After SAH

  作者：Liu Y, Yao C, Sheng B, et al 杂志名称：Translational Stroke Research IF：7

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