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Fig. Standard Curve of Lactate assay. The y-axis is OD450 and the x-axis is Lactate concentration (mM).

• 如果客户样本不能在同一天收集检测，会间隔几天，该样品是Lactate Assay Buffer处理过后冻在-80度吗？

  可以。

• 乳酸检测试剂盒可以检测细胞培养液里的乳酸含量吗？

  可以，但是需要把细胞给分离出来，单独取定量的细胞培养液进行检测。

• 该试剂盒是否可以测乳酸生成？

  可以测，建议分别取生化反应前后的样本，并分别进行检测，计算乳酸含量变化的差值。

• 该试剂盒里面有一个操作是需要去除所有蛋白质，该需要怎么做？

  需要，用过滤除杂的方法，内源性乳酸脱氢酶（LDH）可降解乳酸。含有LDH （如培养基或组织裂解液）的样品应通过10 kDa MW旋转过滤器过滤，以去除所有蛋白质，然后-80°C 保存。

• 该试剂盒检测细胞培养液的前处理的方法是什么？

  以2000g的速度离心5min，取0.5-10 µL上清液可直接用Lactate Assay Buffer稀释到50 µL/孔进行检测。

• 本试剂盒中检测OD0 min的意义是什么？设置OD30 min-OD0 min的作用是什么？

  本试剂盒的OD0 min是指样本溶液中可能存在背景OD值，OD30 min-OD 0min的作用是将检测结果减去背景OD值，消除实验背景。

• 该乳酸检测试剂盒加完buffer之后是颠倒混匀，还是要用移液器吹打混匀呢？

  离心管里面要用斡旋仪斡旋10s。

本产品已在(30)种出版物中被引用

• Circular RNA, circular RARS, promotes aerobic glycolysis of non‐small‐cell lung cancer by binding with LDHA

  作者：Li H, Huang Q, Guo H, Chen X, Li X, Qiu M, 杂志名称：Thorac Cancer IF：3

  查看文献

• 3-tert-Butyl-4-hydroxyanisole perturbs renal lipid metabolism in vitro by targeting androgen receptor-regulated de novo lipogenesis

  作者：Wang, Xiaoyun, et al. 杂志名称：Ecotoxicology and Environmental Safety IF：4

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• Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR …

  作者：Li,Zhuo,et al.  杂志名称：International Journal of Oncology IF：5

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• TNF‐α‐Induced KAT2A Impedes BMMSC Quiescence by Mediating Succinylation of the Mitophagy‐Related Protein VCP

  作者：Su, Zepeng, et al 杂志名称：Advanced Science IF：15

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• Tumor microenvironment-activated Nb2C quantum dots/lactate oxidase nanocatalyst mediates lactate consumption and macrophage repolarization for enhanced …

  作者：Zhang, Yaqian, et al. 杂志名称：Colloids and Surfaces B: Biointerfaces IF：6

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• Tumor microenvironment-activated Nb2C quantum dots/lactate oxidase nanocatalyst mediates lactate consumption and macrophage repolarization for enhanced …

  作者：Zhang, Yaqian, et al. 杂志名称：Colloids and Surfaces B: Biointerfaces IF：5

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• α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure

  作者：Zhang, Naijin, et al. 杂志名称：Cell Research IF：44

  查看文献

• Vitamin D-VDR (vitamin D receptor) alleviates glucose metabolism reprogramming in lipopolysaccharide-induced acute kidney injury

  作者：Dai, Qing, et al. 杂志名称：Frontiers in Physiology  IF：3

  查看文献

• Lactate exacerbates lung damage induced by nanomicroplastic through the gut microbiota–HIF1a/PTBP1 pathway

  作者：Xuan L, Xu Z, Luo J, et al 杂志名称：Experimental & Molecular Medicine IF：13

  查看文献

• α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure

  作者：Zhang, Naijin, et al. 杂志名称：Cell Research IF：44

  查看文献

• FcγRIIIA activation-mediated up-regulation of glycolysis alters MDSCs modulation in CD4+ T cell subsets of Sjögren syndrome

  作者：Qi, Jingjing, et al. 杂志名称：Cell Death & Disease IF：9

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• Peptide-based PROTAC degrader of FOXM1 suppresses cancer and decreases GLUT1 and PD-L1 expression

  作者：  Wang K,  Dai X,  Yu A 杂志名称：Journal of Experimental & Clinical Cancer Research IF：13

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• Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression

  作者：Chengmin Liu, Ke Jiang, Yanyan Ding, Aihua Yang, Renwei Cai, Panzhu Bai 杂志名称：theranostics IF：12

  查看文献

• 3-tert-Butyl-4-hydroxyanisole perturbs renal lipid metabolism in vitro by targeting androgen receptor-regulated de novo lipogenesis

  作者：Wang, Xiaoyun, et al. 杂志名称：Ecotoxicology and Environmental Safety IF：7

  查看文献

• Targeting the PDK/PDH axis to reverse metabolic abnormalities by structure-based virtual screening with in vitro and in vivo experiments

  作者：J Yue, J Xu, Y Yin 杂志名称：International Journal of Biological Macromolecules IF：8

  查看文献

• Vitamin D-VDR (vitamin D receptor) alleviates glucose metabolism reprogramming in lipopolysaccharide-induced acute kidney injury

  作者：Dai, Qing, et al. 杂志名称：Frontiers in Physiology  IF：4

  查看文献

• Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC

  作者：Wang, Kaixuan, et al. 杂志名称：Frontiers in Pharmacology IF：6

  查看文献

• Circular RNA, circular RARS, promotes aerobic glycolysis of non‐small‐cell lung cancer by binding with LDHA

  作者：Li H, Huang Q, Guo H, Chen X, Li X, Qiu M, 杂志名称：Thorac Cancer IF：3

  查看文献

• Lactate exacerbates lung damage induced by nanomicroplastic through the gut microbiota–HIF1a/PTBP1 pathway

  作者：Xuan, L., Xu, Z., Luo, J., Wang, Y., Yan, Y., Qu, C., ... & Huang, R 杂志名称：Experimental & Molecular Medicine IF：13

  查看文献

• Lactate administration induces skeletal muscle synthesis by influencing Akt/mTOR and MuRF1 in non‐trained mice but not in trained mice

  作者：S Kyun, J Kim, D Hwang, I Jang, HY Park, K Lim 杂志名称：Physiological Reports IF：2

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• Chicoric acid ameliorates sepsis-induced cardiomyopathy via regulating macrophage metabolism reprogramming

  作者：Sun H J, Zheng G L, Wang Z C 杂志名称：Phytomedicine IF：8

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• Transcription Factor STAT3-Activated LDHB Promotes Tumor Properties of Endometrial Cancer Cells by Inducing MDH2 Expression

  作者：Shen L, Wang J, Li Y 杂志名称：Molecular Biotechnology IF：3

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• FcγRIIIA activation-mediated up-regulation of glycolysis alters MDSCs modulation in CD4+ T cell subsets of Sjögren syndrome

  作者：Qi, Jingjing, et al. 杂志名称：Cell Death & Disease IF：5

  查看文献

• Metformin Attenuates Neutrophil Recruitment through the H3K18 Lactylation/Reactive Oxygen Species Pathway in Zebrafish

  作者：R Zhou, RC Ding, Q Yu, CZ Qiu, HY Zhang, ZJ Yin, DL Ren 杂志名称：Antioxidants IF：7

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• Tamsulosin alters the pharmacokinetics of metformin via inhibition of renal multidrug and toxin extrusion protein 1 and organic cation transporter 2 in rats

  作者：Saad AAA, Zhang F, Refat M, Mohammed EAH, Zhang M, Chen Y, Al Hamyari B, Alafifi J, Wu X, 杂志名称：J Pharm Biomed Anal IF：3

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• Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR …

  作者：Li,Zhuo,et al.  杂志名称：International Journal of Oncology IF：5

  查看文献

• TAB182 Regulates Glycolytic Metabolism by Controlling LDHA Transcription to Impact Tumor Radiosensitivity

  作者：Chen, S., Xie, D. F., Li, S., Luo, J., Han, Y., Guo, H., ... & Zhou, P. K 杂志名称：Cell Death & Disease IF：9

  查看文献

• Simultaneous suppression of PKM2 and PHGDH elicits synergistic anti-cancer effect in NSCLC

  作者：Wang, Kaixuan, et al. 杂志名称：Frontiers in Pharmacology IF：6

  查看文献

• Modulation of SEMA4D-modified titanium surface on M2 macrophage polarization via activation of Rho/ROCK-mediated lactate release of endothelial cells: In vitro …

  作者：Zhou J, Mei Y K, Qian X 杂志名称：Colloids and Surfaces B: Biointerfaces IF：6

  查看文献

• Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression

  作者：Chengmin Liu, Ke Jiang, Yanyan Ding, Aihua Yang, Renwei Cai, Panzhu Bai 杂志名称：theranostics IF：12

  查看文献